The transportation was simulated of aerosol particles released from point-source over the typical hilly terrain in a wind tunnel. The Particle Dynamic Analysis technique (PDA) was used to investigate the effects of wind speed (1 m/s, 2 m/s), particle size (0.1~2, 2~4, 4~6 μm), and terrain conditions (different height of peaks) on the migration behavior of particles in the range of roughness sub-layers. It was found that the longitudinal velocity and vertical velocity probability distribution of particles in the range of roughness sub-layer are in accordance with the Gaussian distribution. Smaller particles display larger correlation coefficients and so better Gaussian fittings. It is showed that increasing wind speed could make the longitudinal motion of particles more dispersed, and the vertical motion of particles is the joint action of wind speed and terrain. By comparing the mean longitudinal and vertical velocity of particles in the range of roughness sub-layer between smooth surface and hilly terrain, it was found that the mean longitudinal velocities of particles over peaks are smaller than those over smooth surface. It is indicated that the airflow can be hindered in the range of roughness sub-layer by the peaks. The above results provide data basis and support for the study of the migration behavior of aerosol particles in the range of roughness sub-layer.