Although the current developed Blu-ray disc-based technology could realize digitized molecular detection, the result analysis of which depends on computer, not applicable for on-site applications. In order to overcome the above challenges, we herein report a quantitative detection system developed by the embedded technology. In particular, through the transplantation of embedded systems and disc quality diagnosis software, and the development of data processing programs, this system realizes the automatic analysis of bio-discs with this integrated device. It has been utilized for the detection of printed ink dots and the biotin-streptavidin binding reaction on BD surface, preving the reliability and feasibility for biomolecular detection.